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KMID : 0350519920450020761
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Journal of Catholic Medical College
1992 Volume.45 No. 2 p.761 ~ p.780
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Effect of Hydrogen peroxide, Povidone-iodine and Running Load on the Rats’ Knee Joint Cartilage and the Effect of Glucosamine Sulfate on Degenerative Joint Cargilage
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Moon Jeong-Lim
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Abstract
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This study was primarily performed to seasrch for the new animal model of the degenerative arghritis, though there have been several animal models being utilized for various cartilage research works. Several agents such as hydrogen
peroxide(H2O2), steroids, and osmic acid have been used to induce the degenerative arthritis. Clinically 30% povidone-iodine solution and H2O2 are often used as wound irrigation solutions. The povidone-iodine solution which leaves the thin film over the tissue surface might damage the articular cartilage by impairing the cartilage nutrition. Therefore, in this study 30% povidone-iodine solution was used as an arthritis-inducing agent, and H2O2 was used for comparison. Glucosamine sulfate was used to assess its efficacy on the cartilage metabolism. For this study twelve week-old 110 Sprague-Dawley rats, weighing 250g on an average were used; Ten were used as controls without any treatment, and 100 rats were equally divided into 5 groups for the experiment. In group I, 0.05ml of normal saline was injected twice into the right knee joints with one week interval, and running load over the treadmill was added until their sacrifice. In group II, 0.05ml of 2% H2O2 was injected into the right knees with one week interval. In group III, 0.05 ml of 2% H2O2 was injected twice into the right knees with one week interval, and running load was added until their sacrifice. In group IV, 0.05ml of 30% povidone-iodine was injected twice into the right knees with one week interval, and running load was added until their sacrifice. In group V, the same treatment was given as in group III, and then 50mg of glucosamine sulfate was daily given intramusculary to the all rats from one week prior to the intraarticular injection of 2% H2O2 until their sacrifice.
In each experimental group, 5 rats wee sacrificed at 2, 4, 6, and 8 weeks after the first intraarticular injection, respectively, and the medial femoral condyles of the joints were taken for the histopathological studies of the articular cartilage.
@ES The results were as follows: @EN 1) In all experimental group, Wagenh?ser’s stage I or II degenerative changes of articular cartilage were induced. 2) In group I(saline injection and running load group), stage I cartilage degeneration was observed at 8 weeks after saline injection. 3) In group II(H2O2 injection group), stage I cartilage degeneration was observed at 4 and 6 weeks after first H2O2 injection, and then the changes did progress to Wagenh?ser stage II egeneration at 7 weeks after H2O2 injection. 4) Only in group III(H2O2 injection and runing load group), stage II cartilage degeneration was induced even at 6 weeks after H2O2 injection. 5) In group IV(povidone-iodine injection and running load group), stage I cartilage degeneration was observed even at 2 weeks after povidone-iodine injection, and was maintaine up to 6 weeks.The stage II degeneration was observed at 8 weeks. The degeneration in this group did progress slowly even though running load was added because of the relatively high matriceal proteoglycan contents in comparison with other group. 6) In group V(H2O2 injection and running load plus glucosamine sulfate injection group), stage I cartilage degeneration being observed at 4 weeks after H2O2 injection, remained unchanged until 8 weeks. 7) Reduction of safranin-0 stainability was observed in group I, II, III and V before the histological changes were observed. Throughthe results,it was found that 30% povidone-iodine and 2% hydrogen peroxide with runing load can induce degenerative arthritis in rats which can be used as an experimental model of the degenerative arthritis,and that glucosamine sulfate
prevents the progress of cartilage degeneration induced by hydrogen peroxide and running load.
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KEYWORD
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